Analytical Sciences

A Novel Coumarin-based Fluorescent Probe for Recognition of Copper(II) Ions and Its Application in Bioimaging

Yang SUN, Minghao LI, Yang JIAO, and Chunying DUAN

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China

Simple, accurate and real-time analytical methods are required for the detection of metal ions in a complex environment. In the present work, a fluorescent probe CPB based on coumarin was designed for recognizing Cu²⁺ ions. The fluorescence of CPB gradually quenched with increasing concentration of Cu²⁺ ions, due to the interactions between CPB and Cu²⁺ ions. With the addition of Cu²⁺ ions, the emission changes of CPB exhibited a good liner relationship toward the Cu²⁺ ions content in solution. Additionally, CPB could highly selective recognize Cu²⁺ ions among other metal ions in solution. Bearing the selectivity and fluorescence property toward Cu²⁺ ions, CPB was successfully applied to monitoring Cu²⁺ ions in Hela cells and zebrafish.

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