Analytical Sciences

A Colorimetric Immunosensor Based on Hemin@MI Nanozyme Composites, with Peroxidase-like Activity for Point-of-care Testing of Pathogenic E. coli O157:H7

Yan LENG,*,** Shengjun BU,** Zhongyi LI,** Zhuo HAO,** Chengyou MA,*** Xiuxia HE,* and Jiayu WAN**

*School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China
**Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China
***College of Geo-Exploration Science and Technology, Jilin University, Changchun 130026, China

Recently, nanozymes have become a topic of particular interest due to their high activity level, stability and biocompatibility. In this study, a visual, sensitive and selective point-of-care immunosensor was established to test the pathogen Escherichia coli O157:H7 (E. coli O157:H7). Hemin and magainin I (MI) hybrid nanocomposites (Hemin@MI) with peroxidase-mimicking activities were synthesized via a “one-pot” method, involving the simple mixing of an antimicrobial peptide (MI) against E. coli O157:H7 and hemin in a copper sulfate sodium phosphate saline buffer. Hemin@MI nanocomposites integrating target recognition and signal amplification were developed as signal probes for the point-of-care colorimetric detection of pathogenic E. coli O157:H7. Hemin@MI nanocomposites exhibit excellent peroxidase activity for the chromogenic reaction of ABTS, which allows for the visual point-of-care testing of E. coli O157:H7 in the range of 10² to 10⁸ CFU/mL, with a limit of detection of 85 CFU/mL. These data suggest this immunosensor provides accessible and portable assessments of pathogenic E. coli O157:H7 in real samples.

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