Analytical Sciences

Based on Gold Nanoparticles-L-Tyr-Amino Functionalized Mesoporous Materials-Polyphenol Oxidase Modified Biosensor for the Detection of Resorcinol

Tongsheng ZHONG,* Qianqiong GUO,** Xiaoyan ZHU,* Rong LIU,* and Shasheng HUANG**

*College of Materials and Chemical Engineering, Hunan City University, Yiyang, 413000, P. R. China
**College of Life and Environmental Science, Shanghai Normal University, Shanghai, 200234, P. R. China

Nowadays, resorcinol (RC) has been widely applied in the chemical and pharmaceutical industries. However, the electrochemical detection technique of RC still features some significant drawbacks, for instance, a low sensitivity. Hence, in the present work, a glass carbon electrode was developed for the electrochemical detection of RC with good specificity and stability, through modifying the glass carbon electrode (GCE) by polyphenol oxidase (PPO), an NH₂-SBA-15 mesoporous material (NH₂-SBA-15), L-tyrosine (L-Tyr) and gold nano-particles (AuNPs). After being successively modified by AuNPs, L-Tyr, NH₂-SBA-15 and PPO, the constructed PPO/NH₂-SBA-15/L-Tyr/AuNPs/GCE was used to discriminate RC from ions and other common micromolecules, which showed a fairly good specificity and stability. The proposed electrochemical detection method features a linear range of from 0.5 to 21.0 μM with a LOD down to 0.15 μM, revealing a better sensitivity than the existing methods. It is worth mentioning that the proposed PPO/NH₂-SBA-15/L-Tyr/AuNPs/GCE has been successfully used as an electrochemical probe for the RC assay in domestic sewage.

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