Analytical Sciences

Tannic Acid Capped Copper Nanoclusters as a Cost-Effective Fluorescence Probe for Hemoglobin Determination

Sonia SAM, Sanu K. ANAND, Manna Rachel MATHEW, and Krishnapillai Girish KUMAR

Department of Applied Chemistry, Cochin University of Science and Technology, Kochi-682022, Kerala, India

For the first time, we report on a copper nanoclusters based fluorescence sensor for hemoglobin (Hgb). The aggregation-induced quenching of tannic acid capped copper nanoclusters’ (TACuNCs) fluorescence by a Hgb-H₂O₂ mixture that mimics the Fenton’s reagent is used here for the selective determination of Hgb. It is possible to effectively determine Hgb using this sensitive and cost-effective sensor in the linear range of 5.0 × 10⁻⁸ to 4.0 × 10⁻⁹ M with a detection limit of 5.6 × 10⁻¹⁰ M. The practical utility of the sensor is evident from the good recovery values obtained from Hgb spiked with artificial blood serum.

J-STAGE: View this article in J-STAGE