Analytical Sciences

Rapid Detection of Dezocine in Biological Fluids Based on SERS Technology

Siqingaowa HAN,*,** Chen ZHANG,* Zhijie CHEN,*** Xuanyu SHA,* and Wuliji HASI*

*National Key Laboratory of Science and Technology on Tunable Laser, Harbin Institute of Technology, Harbin 150080, China
**Affiliated Hospital of Inner Mongolia University for the Nationalities, Inner Mongolia Tongliao 028007, China
***Department of Ophthalmology, Harbin 242 Hospital, Harbin 150066, China

This paper describes a method based on surface enhanced Raman spectroscopy (SERS) technology for rapid detection of dezocine in urine and serum. Firstly, an Ag colloid substrate was prepared and characterized. Then the Raman characteristic peaks of dezocine were assigned from both theoretical and experimental aspects. Finally, the Raman peak at 661 cm⁻¹ was selected as its characteristic peak to perform SERS detection on dezocine in urine and serum, and the detection limits of dezocine in urine and serum were determined. The relationships between the characteristic peak intensity and the concentration of dezocine in urine and serum were fitted and the recovery rates were calculated. This rapid, accurate and non-destructive method establishes a good foundation for rapid on-site detection of dezocine in biological samples.

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