Analytical Sciences

Establishment of Detection Methods for Five Cannabinoids in Hemp Cosmetics Based on HPLC

Dong-Mei SUN,*1,*2 Lun SONG,*3 Hai-Yan WANG,*4 Cong-Cong YU,*1 Qiao CHU,*1 Tao LAN,*1 and Wei-Bing ZHANG*2

*1 Sub-Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing 100191, China
*2 East China University of Science and Technology, Shanghai 200237, China
*3 Shanghai Yuansi Standard Science and Technology Co., Ltd., Shanghai 200120, China
*4 National Institutes for Food and Drug Control, Beijing 100050, China

A simultaneously HPLC detection method for cannabidiolic acid (CBDA), cannabidiol (CBD), cannabinol (CBN), Δ9-tetrahydrocannabinol (THC), tetrahydro-cannabinolic acid (THCA) in 3 kinds of cosmetics matrix containing hemp leaf extract was developed. The extraction and HPLC conditions were optimized, and a methodological verification was also carried out. The results showed that this method had a good linear relationship in the range of 0.25 – 50 μg/mL with LOD values for 5 cannabinoids all between 0.10 – 0.25 μg/g. The recovery rates of 5 cannabinoids in 3 different cosmetics matrixes were between 90.1 – 108.5%, and the RSD values were all below 4.4%. These results indicated that this method had the advantages of simple operation, high sensitivity, and good accuracy. Through the testing of 6 kinds of hemp cosmetics, it was found that such cosmetics had uneven quality. The establishment of this method can lay a methodological foundation for establishing relevant testing method standards.

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