Analytical Sciences

Digestion Efficiency during Alkaline Persulfate Oxidation for Determination of Total Phosphorus Content of Biological Samples

Yuji ONISHI

Center for Ecological Research, Kyoto University, 2-509-3 Hirano, Otsu, Shiga 520-2113, Japan

Quantifying total phosphorus contents of organisms can elucidate their physiological condition and the nutrient cycles of ecosystems. Simple, brief, and safe persulfate oxidation methods have been used for total P determination, but oxidizing solutions of different compositions and volumes have been used. Two certified reference materials were used to evaluate digestion efficiencies of different solutions for this study. Although the phosphorus recoveries were low (<90%) without NaOH, phosphorus recoveries using the solution with 4% K₂S₂O₈ and 0.15 M NaOH were complete. Results demonstrated that digestion efficiency depends on the K₂S₂O₈ concentration and on the pH condition. Moreover, the phosphorus recoveries were achieved at >4 mL/mg solution/material ratios for both standard materials. Therefore, the author recommends using >4 mL of the 4% K₂S₂O₈ solution with 0.15 M NaOH for sample materials of <1 mg to quantify the total phosphorus of biological samples.

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