Abstract − Analytical Sciences, 37(11), 1491 (2021).
TLC-based MS Imaging Analysis of Glycosphingolipids and Glycerin Fatty Acid Esters after 1,2-Dichloroethane Washing
Shoko MATSUSHITA, Takuma HASEGAWA, Marina HIRAOKA, Aki HAYASHI, and Yusuke SUZUKI
Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, 228A College of Science and Technology Building No. 2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo 101-0062, Japan
Matrix-assisted laser desorption/ionization-based mass spectrometry imaging (MSI) of separated lipids on thin-layer chromatography (TLC) plates or followed by blotted hydrophilic polyvinylidene fluoride (PVDF) membranes has become a powerful tool in lipidomic analyses. However, background peaks in MS spectra often cover lipid peaks in a low amount/ionization effect; consequently, only low intensities/resolutions MSI are obtained. To address the aforementioned problem, we attempted 1,2-dichloroethane pre-washing of TLC plates before development and found that backgrounds could successfully be removed from the TLC plate or PVDF membrane.
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