Analytical Sciences

Crumpled Graphene/Poly (azure I) Modified Electrode for Non-enzymatic Detection of Hydrogen Peroxide Secreted from Tumor Cells

Keying ZHANG,*,** Ziqiang ZHANG,* Shangshang MA,* Deshun CHENG,* Mengdi FAN,* Xinyu ZHOU,* Fajun LI,* and Na ZHANG*

*Anhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, Anhui 234000, China
**State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China

Hydrogen peroxide (H₂O₂), an important representatives of reactive oxygen species, its aberrant expression is related to many diseases, including cancers. Therefore, it is very significant to design a reliable method for the real-time detection of endogenous H₂O₂. Herein, we describe preparing a poly (azure I)/crumpled graphene (cGN) modified electrode by an electro-polymerization method. The results showed that this electrode presented obvious electrocatalytic effect on the reduction of H₂O₂. Amperometric method was employed to monitor H₂O₂, and the amperometric response exhibited a good linear relationship with its concentration in the range of 8.0 × 10⁻⁶ – 1.25 × 10⁻³ mol/L; the detection limit reached to 6.7 × 10⁻⁷ mol/L (S/N = 3). Furthermore, this modified also displayed good selectivity, long-term stability and a high anti-interference ability. Excitingly, the established method could be successfully used to detect H₂O₂ in human serum samples, and measured H₂O₂ secreted from living MCF-7 cells. It could have potential use in cancer diagnosis.

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