Analytical Sciences

Structural Characterization of Proteins Adsorbed at Nanoporous Materials

Akira YAMAGUCHI,* Masahiro SAIGA,* Daiki INABA,* Mami AIZAWA,* Yuta SHIBUYA,** and Tetsuji ITOH***

*Institute of Quantum Beam Science, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan
**New Industry Creation Hatchery Center, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan
***National Institute of Advanced Industrial Science and Technology (AIST), 4-2-1 Nigatake, Miyagino, Sendai 983-8551, Japan

A nanoporous material has been applied for the development of functional nanobiomaterials by utilizing its uniform pore structure and large adsorption capacity. The structure and stability of biomacromolecules, such as peptide, oligonucleotide, and protein, are primary factors to govern the performance of nanobiomaterials, so that their direct characterization methodologies are in progress. In this review, we focus on recent topics in the structural characterization of protein molecules adsorbed at a nanoporous material with uniform meso-sized pores. The thermal stabilities of the adsorbed proteins are also summarized to discuss whether the structure of the adsorbed protein molecules can be stabilized or not.

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