Analytical Sciences

Preparation of a Sandwich-like Complex “MIPs-Target Molecule-Magnetic SERS Probe” and SERS Determination of Immunoglobulin G

Xiaoxuan LI,* Lijun LI,** and Yuhan HE*

* Province and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry, Nanning 530004, Guangxi, P. R. China
**Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, P. R. China

Based on the reversible covalent binding between borates and glycoproteins, we proposed a scheme that can specifically recognize and detect immunoglobulin G (IgG). The application of magnetic materials to enrich SERS probes can provide an enhanced signal and repeatability. The results showed that the prepared sandwich-like complex displayed high sensitivity and excellent selectivity for IgG. The Raman peak intensity showed a good linear relationship in the concentration range of 1 mg/mL – 1 ng/mL. The linear equation was y = 657.93x + 2963.9, R² = 0.996. Parallel testing of this sandwich-like complex proved to have excellent repeatability. This method provided a new possibility for clinical non-immunity and the quantitative detection of IgG.

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