Analytical Sciences

Untargeted Lipidomic Analysis of Plasma from High-fat Diet-induced Obese Rats Using UHPLC–Linear Trap Quadrupole–Orbitrap MS

Siddabasave Gowda B. GOWDA,*1 Zi-Jun GAO,*1 Zhen CHEN,*1 Takayuki ABE,*2 Shota HORI,*2 Satoru FUKIYA,*3 Satoshi ISHIZUKA,*3 Atsushi YOKOTA,*3 Hitoshi CHIBA,*4 and Shu-Ping HUI*1

*1 Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita, Sapporo 060-0812, Japan
*2 Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita, Sapporo 060-8589, Japan
*3 Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita, Sapporo 060-8589, Japan
*4 Department of Nutrition, Sapporo University of Health Sciences, Nakanuma, Nishi-4-3-1-15, Higashi, Sapporo 007-0894, Japan

High-fat diet (HFD)-induced obesity is a primary risk factor for serious health problems. Although much research has been performed at the genomic level, lipidomic studies were limited. In this study, we aim to obtain a comprehensive profile of circulating plasma lipids, which are altered in rodent rat obesity by untargeted liquid chromatography–mass spectrometry. Rats fed with HFD for 8 weeks had increased body weight, liver and adipose tissue weight. The analysis results revealed that polyunsaturated fatty acids (PUFAs) and their corresponding phosphatidylcholine, phosphatidylinositol, and phosphatidylserine were significantly decreased in rats fed with HFD. In contrast, less unsaturated and ether type phosphatidylglycerols were increased. The triacylglycerides (TAGs) having saturated FA were increased in the HFD condition, whereas TAGs having PUFA were decreased. The levels of many plasma lipids were altered, and interestingly PUFA derived lipids were negatively associated with obesity. This signifies the importance of a PUFAs enriched diet to overwhelm obesity associated diseases.

J-STAGE: View this article in J-STAGE