Analytical Sciences

Fluorescence Enhancement Effect by Metal Nanoparticles-immobilized Microplate

Shuyi SUN,* Kyohei MATSUI,* So TANABE,* Dung NGUYEN,* Takamasa KINOSHITA,* Yojiro YAMAMOTO,*,** and Hiroshi SHIIGI*

*Department of Applied Chemistry, Osaka Prefecture University, 1-2 Gakuen, Naka, Sakai, Osaka 599-8570, Japan
**GreenChem. Inc., 19-19 Tsuruta, Nishi, Sakai, Osaka 593-8323, Japan

In this reported work, we achieved high-throughput, highly sensitive fluorescent analysis using an enzyme-linked immunosorbent assay (ELISA) that employed a metallic nanoparticle (NP)-immobilized 96-well plate. The immobilization of metallic NPs on a 96-well plate effectively amplified fluorescent signals of the assay. The silver (Ag) NP-immobilized plate showed the best fluorescent enhancement effect of all plates immobilized by metal NPs. Our results demonstrate the potential of applying Ag NPs to enhance the efficiency of direct and indirect ELISA by the labeling of targets.

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