Analytical Sciences

Simultaneous Detection of Multiple β-Adrenergic Agonists with 2-Directional Lateral Flow Strip Platform

Qian WU,* Qing SONG,* Xinxin WANG,* Li YAO,* Jianguo XU,* Jianfeng LU,* Guodong LIU,** and Wei CHEN*,**

*Engineering Research Center of Bio-process, MOE, School of Food Science & Engineering, Hefei University of Technology, Hefei, 230009, China
**Research Center for Biomedical and Health Science, School of Life and Health, Anhui Science & Technology University, Fengyang, 233100, China

Clenbuterol (CL), salbutamol (SAL) and ractopamine (RAC) are the three common β-adrenergic agonists, which are the main hazards in food safety and affect human health through the food chain. A convenient and efficient method is urgently required to perform on-site detection of multiple β-adrenergic agonists to avoid frequent poisoning incidents. In this paper, a 2-directional lateral flow strip technique (2-directional LFS) is developed for rapid and simultaneous detection of CL, SAL and RAC with single sampling. Compared to the conventional lateral flow strip, this 2-directional LFS technique can realize simultaneous detection of three or more target analytes without any change of intrinsic simplicity of LFS. Furthermore, this 2-directional LFS can effectively avoid the potential intrinsic cross-reactivity among the reagents to analogues. Under the optimized conditions, CL, SAL and RAC were all successfully determined with satisfactory results in both buffer and urine samples with the detection limit as low as 0.5 ng/mL. This 2-directional LFS technique can revolutionize the commercial single-analyte LFS products and can effectively widen the applications of the classic LFS in various fields.

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