Analytical Sciences

A RuO₂ Nanosheet as a Novel Quencher-free Platform for the Detection of Nucleic Acids in a Homogeneous Solution

Yusuke KITAMURA,* Takaaki TANIGUCHI,** Miwako TSUTSUMI,* Leanddas NURDIWIJAYANTO,** Tomoya MATSUO,* Yousuke KATSUDA,* and Toshihiro IHARA*

*Division of Materials Science, Faculty of Advanced Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo, Kumamoto 860-8555, Japan
**World Premier International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

A fluorescent dye-labeled DNA probe was adsorbed and quenched on the monolayer of RuO₂ nanosheets. Significant fluorescent recovery was observed upon the addition of complementary DNA due to desorption of the probe from the surface of the RuO₂ nanosheet through duplex formation. The efficiency of fluorescence recovery was higher than that for graphene oxide, which was known as a quencher-free platform for the detection of nucleic acids in a homogeneous solution.

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