Analytical Sciences

Simultaneous Detection of Six Different Types of Pesticides by an Immunosensor Based on Surface Plasmon Resonance

Shiro MIYAKE,*1,*2,*3 Yuki HIRAKAWA,*1,*4 Tomomi YAMASAKI,*1,*4,*5 Eiki WATANABE,*6 Ayako HARADA,*7 Seiji IWASA,*7 and Hiroshi NARITA*4

*1 Advanced Science, Technology & Management Research Institute of Kyoto, Shimogyo, Kyoto 600-8813, Japan
*2 Advanced R&D Center, HORIBA, Ltd., Minami, Kyoto 601-8510, Japan
*3 Azabu University, Sagamihara, Kanagawa 252-5201, Japan
*4 Kyoto Women’s University, Higashiyama, Kyoto 605-8501, Japan
*5 Osaka Institute of Public Health, Higashinari, Osaka 537-0025, Japan
*6 Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8604, Japan
*7 Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan

Six pesticides, azoxystrobin, boscalid, chlorfenapyr, imazalil, isoxathion, and nitenpyram, were simultaneously detected by using a surface plasmon resonance (SPR) immunosensor. The working ranges were 3.5 – 19 ng/mL for azoxystrobin, 4.5 – 50 ng/mL for boscalid, 2.5 – 25 ng/mL for chlorfenapyr, 5.5 – 50 ng/mL for imazalil, 3.5 – 50 ng/mL for isoxathion, and 8.5 – 110 ng/mL for nitenpyram. They showed adequate recovery results in tomato samples: 104 – 116% for azoxystrobin, 94 – 101% for boscalid, 90 – 112% for chlorfenapyr, 96 – 106% for imazalil, 107 – 119% for isoxathion, and 104 – 109% for nitenpyram. The correlation coefficient with liquid chromatography (HPLC or LC-MS/MS) using vegetable samples also agreed well: 0.91 – 0.99 as R² without strong bias, except for nitenpyram for which the SPR immunosensor sensitivity was too low. The SPR immunosensor will have high applicability for pesticide residue analyses in vegetable samples.

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