Analytical Sciences

Nanocellulose Films to Improve the Performance of Distance-based Glucose Detection in Paper-based Microfluidic Devices

Sangkaew PRAPAPORN,* Sriruangrungkamol ARISARA,** Chonkaew WUNPEN,** and Dungchai WIJITAR*

*Organic Synthesis, Electrochemistry & Natural Product Research Unit, Department of Chemistry, Faculty of Science, King Mongkut’s University of Technology Thonburi, Prachautid Road, Thungkru, Bangkok, 10140, Thailand
**Department of Chemistry, Faculty of Science, King Mongkut’s University of Technology Thonburi, Prachautid Road, Thungkru, Bangkok, 10140, Thailand

We report on a simple, cost-effective, instrument-free, and portable distance-based paper device coupled with NFs for the determination of glucose. The analysis reaction is based upon the oxidative etching reaction of silver nanoparticles (AgNPs) in the presence of H₂O₂ that is produced from glucose after a glucose oxidase (GOx) catalytic reaction leading to a morphological transformation of AgNPs. A color band length of AgNPs is coated on to a detection channel and then etched by H₂O₂, and these were changed from a purple color to colorless as a correlate of the glucose concentration. To improve the performance of the enzyme immobilization, NFs, which are biocompatible without compromising their structure and biological activity, were then placed onto the sample zone. The naked-eye detection limit was 0.1 mM for 40 min of analysis time. The recoveries of glucose spiked in the artificial urine samples and control urine samples were then verified by our device and were in the acceptable range of 96 – 100%.

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