Analytical Sciences

Surface-enhanced Raman Spectroscopy Coupled with Dispersive Solid-phase Extraction for the Rapid Detection of Tricyclazole Residues in Rice and Brassica campestris L. ssp. chinensis var. utilis Tsen

Wenjun ZHANG,* Zhen LIU,** Hongwei QIN,* Huidong LI,* Hongxia DU,* Liping FANG,* Chenchen WANG,* Shuqiu ZHANG,* and Zilei CHEN*

*Institute of Quality Standard and Testing Technology for Agro-products, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
**Institute for Advanced Interdisciplinary Research (IAIR), School of Materials Science and Engineering, University of Jinan, Ji’nan, China

An efficient methodology has been developed to determine the tricyclazole residue in matrix based on surface-enhanced Raman scattering (SERS) coupled with dispersible matrix solid-phase extraction. After pretreatment and test conditions optimization, peaks at 1373 and 1317 cm⁻¹ in the SERS spectrum were respectively selected as quantitative peaks for rice and Brassica campestris L. ssp. chinensis var. utilis Tsen, respectively. The matrix standard curve-external standard method was used to quantitatively conduct a statistical analysis. The correlation between the quantitative peak response and tricyclazole concentration showed a significant linear relationship with a correlation coefficient of R² > 0.99. The lowest spiked concentration was determined to be the quantitative limit that was below the maximum residue limits of tricyclazole. This study provides a sensitive, stable and rapid approach for the analysis of tricyclazole in above matrix via SERS, and it will be a useful complement to the quantitative analysis of tricyclazole in a complex matrix.

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