Analytical Sciences

Simultaneous Determination of Aminoglycoside Residues in Livestock and Fishery Products by Phenylboronic Acid Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Yoshiaki FUJII, Takero KAGA, and Kazuhiko NISHIMURA

Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita, Sapporo, Hokkaido 060-0819, Japan

A rapid and simple method has been developed for simultaneous determinations of the concentrations of nine aminoglycosides (AGs) in livestock and fishery products using phenylboronic acid (PBA) solid-phase extraction (SPE) clean-up. Unlike the widely employed SPE approaches, based on cation-exchange, PBA SPE relies on the reversible formation of covalent bonds with the analytes. The advantage of using PBA lies in the fact that this compound strongly and stably retains analytes, and the pH of the loading solution can be easily adjusted using a high-concentration buffer. The clean-up conditions, such as the pH of the loading solution and the acetonitrile concentration in the elution and wash solvents, were optimized. The degree of recovery measured for nine AGs in six samples (bovine muscle, bovine liver, milk, chicken egg, fish and shrimp) were in the 73 – 98% range, and the values for the relative standard deviation were 9.3% or less.

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