Analytical Sciences

Abstract − Analytical Sciences, 35(9), 1037 (2019).

Comparative Study of Electrochemical Sensors Based on Enzyme Immobilized into Polyelectrolyte Microcapsules and into Chitosan Gel
Yulia V. PLEKHANOVA,*1 Sergei A. TIKHONENKO,*2 Alexey V. DUBROVSKY,*2 Alexander L. KIM,*2,*3 Egor V. MUSIN,*2,*3 Gou-Jen WANG,*4 Iren E. KUZNETSOVA,*5 Vladimir V. KOLESOV,*5 and Anatoly N. RESHETILOV*1,*5
*1 PSCBR RAS G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia
*2 FSBIS Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya St. 3, Pushchino, Moscow Region 142290, Russia
*3 Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, Russia
*4 Department of Mechanical Engineering, National Chung-Hsing University, Taichung 402, Taiwan
*5 A. Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Mokhovaya St. 11-7, Moscow 125009, Russia
The characteristics of an electrochemical biosensor based on a Prussian-blue screen-printed electrode containing glucose oxidase incorporated into polyelectrolyte microcapsules (PMC) are considered. PMC with the embedded enzyme were formed using sodium polystyrene sulfonate and poly(allylamine hydrochloride). The characteristics were compared with those of the enzyme immobilized in chitosan gel. We assessed the dependences of biosensor signals on the composition of the buffer solution, on the glucose concentration; the operational and long-term stabilities. The enzyme immobilized in PMC proved to be more sensitive to buffer molarity at a maximum within 35 – 40 mM. The apparent Michaelis constants were 1.5 and 4.1 mM at the immobilization in, respectively, chitosan and PMC. The developed biosensors were used to assay commercial juices. The biosensors’ data on the glucose contents were shown to have a high correlation with the standard spectrophotometric assay (0.92 – 0.95%), which implies a possible application of the fabricated biosensors in foodstuff analysis.