Analytical Sciences

A Convenient Electrochemiluminescent Immunosensor for Detecting Methamphetamine Antibody

Xia WANG,* Suyan ZHAI,** Chao LIU,** Xiaoshu WANG,** Ya YANG,** and Yifeng TU*

*College of Chemistry, Chemical Engineering and Materials, Dushu Lake Campus, Soochow University, Industrial Park, 215123, Suzhou, P. R. China
**Department of Forensic Medicine, Institute of Forensic Science, Dushu Lake Campus, Soochow University, Industrial Park, 215123, Suzhou, P. R. China

An antibody-based immunotherapy for methamphetamine (MA) addictive treatment is has been drawing more and more attention in recent years. However, studies about methamphetamine antibody (anti-MA) immunodetections are rare, owing to the lack of immunogenicity of small molecule MA. This study provides a simple and effective approach to develop a convenient electrochemiluminescent (ECL) immunosensor for the testing of anti-MA. In short, the synthetic holoantigen of MA is immobilized on a homemade gold nanoparticles modified electrode as the sensing host for the specific recognition and detection of anti-MA. The research suggested, under optimal experimental conditions, the ECL intensity on resultant immunosensor has a wide-linear regression toward the anti-MA quantity within the range from 0.03 to 3.07 ng with a detection limit of 2.32 pg. It responded to the dosage of anti-MA in spiked blood samples with satisfactory recovery. According to the research, the developed sensor shows promise as a portable Anti-MA fast seized device which performs quickly and offers convenience, and will be helpful for forensic identification and clinical treatment.

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