Analytical Sciences

Assessment of Concentrations of Four Phenothiazine Antipsychotics in Serum and Whole Blood Using Different Diatomaceous Earth-based Solid-phase Columns: A Comparative Analysis

Takeshi SAITO,* Akira NAMERA,** Tomoatsu TSUJI,* and Sadaki INOKUCHI*

*Department of Emergency and Critical Care Medicine, Tokai University School of Medicine, Kanagawa 259-1143, Japan
**Department of Forensic Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan

This study attempted to determine the phenothiazine antipsychotics concentration in serum and whole blood samples using various diatomaceous earth-based solid-phase columns and elution solvents and subsequently evaluate their efficiency. Phenothiazine antipsychotics concentrations of 5 – 2000 ng/mL were extracted from serum and whole blood using each column. All compounds were analyzed using liquid chromatography–tandem mass spectrometry. Phenothiazine antipsychotics extraction in serum and whole blood using diatomaceous earth-based solid-phase columns seemed to have an affinity with the elution solvent.

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