Analytical Sciences

Development of Tube Radial Distribution Chromatography Based on Phase-Separation Multiphase Flow Created via Pressure Loss

Susumu WADA,* Kazuhiko TSUKAGOSHI,* Katsumi TSUCHIYA,* Ken HIROTA,* Kenichi YAMASHITA,** and Masaharu MURATA***

*Applied Chemistry, Graduate School of Science and Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan
**Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 807-1 Shuku-machi, Tosu, Saga 841-0052, Japan
***Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi, Fukuoka 812-8582, Japan

A tube radial distribution chromatography (TRDC) method based on phase-separated multiphase flow created through phase transformation via temperature change has been developed. These systems typically required a temperature-controlling device containing a water bath and a stirrer. Herein, we proposed a novel TRDC system without a cooling device, where the phase transformation was achieved via pressure loss in a capillary tube of 50 μm inner diameter and 550 cm length. Model analytes were successfully separated with the developed TRDC system, which provided a simplified platform and helped to clarify the operating principle of TRDC based on phase transformation in a capillary tube.

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