Analytical Sciences

Rapid Formation of Arrayed Cells on an Electrode with Microwells by a Scanning Electrode Based on Positive Dielectrophoresis

Hiroki OKAYAMA,* Masahiro TOMITA,** Masato SUZUKI,* and Tomoyuki YASUKAWA*

*Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan
**Division of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurima-Machiya-cho, Tsu, Mie 514-8507, Japan

We have developed a simple and rapid formation of a cell-based array on microwell array electrodes by an attractive force of positive dielectrophoresis (p-DEP), even after removing an upper disk electrode stick that was used as a counter electrode to the microwell array electrodes. The attractive force of p-DEP generated by the scanning of the disk electrode allows the formation of a cell-based array on all microwell arrays. We demonstrated an exploration of target cells spiked with a low ratio after removing the disk electrode.

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