Analytical Sciences

A Conjugated Polymer Fluorescent Sensor for Continuous Identification of Copper(II) and Pyrophosphate in Blood Serum and Synovial Fluid

Mingming GUAN,* Chunling XU,** Jiahua MA,*** Ting YANG,* Jilin LIU,* and Guodong FENG*

*Department of Analytical Chemistry, College of Chemistry, Jilin University, Jiefang 2519, Changchun 130012, P. R. China
**Department of Ophthalmology, The Second Hospital of Jilin University, Changchun 130041, P. R. China
***Engineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Southwest University of Science and Technology, Mianyang 621010, P. R. China

A novel “on-off-on” super-sensitive conjugated polymer fluorescence sensor (PPE-DPA) was developed and it was applied to realize the continuous recognition of Cu²⁺ and pyrophosphate (PPi). The fluorescence intensity decreased linearly with the change of Cu²⁺ from 0.05 to 5.0 μmol L⁻¹ and the limit of detection was 24 nmol L⁻¹. The fluorescence intensity was linearly enhanced with the increase of PPi from 0.5 to 12.0 μmol L⁻¹ and the limit of detection was 230 nmol L⁻¹. In addition, this method was applied to detect PPi in the blood serum and synovial fluid of patients with arthritis and satisfactory results were obtained. Thus, the PPE-DPA is not only an effective tool for detecting Cu²⁺ and PPi in samples, but also presents a potential way to diagnose arthritis.

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