Analytical Sciences

Rapid Detection of the Bursaphelenchus Xylophilus by Denaturation Bubble-mediated Strand Exchange Amplification

Caiyan LIU,*1 Zengjuan HU,*2 Xiong WANG,*1 Yilong GENG,*3 Cuiping MA,*4 Zonghua WANG,*1 Ronggui LI,*1 and Chao SHI*1

*1 College of Chemistry and Chemical Engineering, College of Life Sciences, Qingdao University, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao University, Shandong, 266071, PR China
*2 Qingdao Agricultural Broadcast and Television School, Qingdao, Shandong, 266109, PR China
*3 Forestry Bureau of Qingdao, Qingdao 266061, PR China
*4 Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Shandong, 266042, PR China

Bursaphelenchus xylophilus (B. xylophilus) is one of the most important causal agents of infectious diseases in forest pathology. Obviously, the rapid detection of B. xylophilus is an urgent need for its prevention and cure. We have developed a detection method of B. xylophilus by strand exchange amplification (SEA). This method could detect 10⁵ copies of genomic DNA of B. xylophilus, and it was sufficiently sensitive to detect a single nematode as short as 40 min. Moreover, because the amplification result could be visualized by the naked eyes, the only equipment required throughout the process was a simple isothermal block. Therefore, our method would be a potential for developing on-site detection of B. xylophilus to prevent and control its spread.

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