Analytical Sciences

Optical Sensing Platform for the Colorimetric Determination of Silver Nanoprisms and Its Application for Hydrogen Peroxide and Glucose Detections Using a Mobile Device Camera

Chutiparn LERTVACHIRAPAIBOON,* Takuya MARUYAMA,* Akira BABA,* Sanong EKGASIT,** Kazunari SHINBO,* and Keizo KATO*

*Graduate School of Science and Technology, Niigata University, 8050 Ikarashi 2-Nocho, Nishi, Niigata 950-2181, Japan
**Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Rd. Patumwan, Bangkok 10330, Thailand

Silver nanoprisms (AgNPrs) have a unique localized surface plasmon resonance, resulting in strong absorption and scattering within the visible light region. In this work, we propose image acquisition from colloidal solutions of AgNPrs using a combination of transmitted and scattered light. The developed measurement technique could be carried out by separately recording transmitted and scattering images of the solutions, using a mobile device camera prior to a calculation of the empirical absorption value (I_A). The I_A value of green for AgNPrs solutions was found to be in agreement with the absorption spectra obtained using a conventional spectroscopic technique. This technique was utilized for the quantifications of hydrogen peroxide and glucose. Good linearities between ΔI_A and those typical analytes were observed. The limit of detection for the typical biosensor of glucose was 19.8 μM. As such, we expect the methodology herein developed for hydrogen peroxide and glucose determinations by means of monitoring the color change of transmitted and scatting images from solutions to contribute to the development of simple, rapid, and reliable detection systems to be further applied to biochemical analysis and clinical diagnosis, as well as to household biosensor applications.

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