Analytical Sciences

Microplate-based Assay for Screening of Advanced Glycation End Products Binding to Its Receptor

Deepak GANESH,* Kyoko TORIGOE,** Miyuki KUMANO-KURAMOCHI,** Sachiko MACHIDA,** and Toshiro KOBORI*,**

*Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
**Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kan-nondai, Tsukuba, Ibaraki 305-8642, Japan

Advanced Glycation End products (AGEs) are a group of amino-acid modifications produced with sugars or di-carbonyls. Some AGEs are known to affect health through binding to the receptor of AGEs (RAGE). Here, we propose a method for screening RAGE-binding AGEs by a competitive assay using purified RAGE and AGEs-specific antibody. This method has clarified that at least carboxyethyl lysine and pentosidine among methylglyoxal-derived AGEs are involved in RAGE binding, suggesting that this would be a promising method for classifying RAGE-binding AGEs.

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