Abstract − Analytical Sciences, 35(12), 1291 (2019).
Development of a Sensitive Chemiluminescent Immunoassay for the Determination of 3-Methyl-quinoxaline-2-carboxylic Acid and Quinoxaline-2-carboxylic Acid in Edible Animal Tissues Using Immunomagnetic Beads Capturing
Yaqing ZHANG,* Song ZHOU,* Xiaoxi CHANG,* and Xiaoqi TAO*,**
*College of Food Science, Southwest University, Chongqing, 400715, China
**Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, Southwest University, Chongqing, 400715, China
**Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, Southwest University, Chongqing, 400715, China
We have reported a sensitive chemiluminescent competitive indirect enzyme-linked immunosorbent assay (CL-ciELISA) based on immunomagnetic beads separation, purification and enrichment for the simultaneous determination of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) and quinoxaline-2-carboxylic acid (QCA) in edible animal tissues. Forty field samples were analyzed with the developed CL-ciELISA, and the results correlated well with those obtained in liquid chromatography–tandem mass spectrometry (LC-MS/MS), confirming the utility of CL-ciELISA for the quantitation of MQCA and QCA in fish, shrimp, pork and chicken with a good accuracy.
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