Analytical Sciences

Array-based Generation of Response Patterns with Common Fluorescent Dyes for Identification of Proteins and Cells

Masahiro MIMURA,*,** Shunsuke TOMITA,** Ryoji KURITA,*,** and Kentaro SHIRAKI*

*Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan
**Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) and DAILAB, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan

A differential array consisting of commercially available common fluorescent dyes was constructed for the identification of proteins and human cancer cells. Fluorescence of dyes was differently altered by mixing with proteins and human cancer cells, generating response patterns that are unique to the analytes. Linear discriminant analysis of the obtained patterns enabled the accurate identification of eight proteins and three human cancer cells. As this system can be easily prepared, it would offer a unique opportunity for array-based differential biosensing.

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