Analytical Sciences

Synthesis of a Peptide-Human Telomere DNA Conjugate as a Fluorometric Imaging Reagent for Biological Sodium Ion

Shinobu SATO,*,** Yuuki IMAICHI,** Yukiko YOSHIURA,*** Koji NAKAZAWA,*** and Shigeori TAKENAKA*,**

*Research Center for Bio-microsensing Technology, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata, Kitakyushu, Fukuoka 804-8550, Japan
**Department of Applied Chemistry, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata, Kitakyushu, Fukuoka 804-8550, Japan
***Graduate School of Environmental Engineering, The University of Kitakyuhsu, 1-1 Hibikino, Wakamatsu, Kitakyushu, Fukuoka 808-0135, Japan

A peptide-oligonucleotide conjugate (1) was synthesized by the attachment of FAM, TAMRA, and biotin moieties to a telomere DNA sequence of 5′-TAG GGT TAG GGT TAG GGT TAG GG-3′. This conjugate was induced to be an anti-parallel structure in the presence of sodium ion (Na⁺), whereas a hybrid one was formed under potassium ion (K⁺) as a monitoring by circular dichromic spectra. The conformation change of this conjugate gave an effective FRET signal change upon the addition of NaCl, compared with the case of KCl. Under 5 mM KCl as an extracellular condition, a FRET change was observed upon addition of NaCl and quantitative FRET change was observed in 0 – 250 mM NaCl. This conjugate was immobilized on the cell surface through a sugar chain on the cell, biotinyl concanavallin A and streptavidin. This conjugate was utilized for Na⁺ sensing based on anti-parallel tetraplex formation with Na⁺.

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