Analytical Sciences

Screening of DNA Signaling Aptamer from Multiple Candidates Obtained from SELEX with Next-generation Sequencing

Toru YOSHITOMI,* Fumiya WAYAMA,* Keiko KIMURA,* Koji WAKUI,* Hitoshi FURUSHO,** and Keitaro YOSHIMOTO*,***

*Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan
**Chemical General Division, Nissan Chemical Industries, Ltd., 2‐10‐2 Tsuboi‐nishi, Funabashi, Chiba 274-8507, Japan
***JST, PRESTO, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan

Here, we demonstrated a strategy for developing signaling aptamers, based on screening of signaling aptamers from multiple aptamer candidates obtained by SELEX with next generation sequencing. Among aptamer candidates labelled by 6-carboxyfluorescein and quencher at both end termini, there is the possibility of discovering a potent signaling aptamer. In this study, we discovered DNA signaling aptamers against VEGFR-1. This strategy has the potential for signaling aptamer discovery without the extremely laborious task of optimization of oligodeoxynucleotide modifications.

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