Abstract − Analytical Sciences, 35(1), 107 (2019).
A Novel Proof-of-Concept Sandwich Immunoassay for Screening Microcystin in Cyanobacteria Based on Michael Addition Reaction
Chenlin HU* and Jiyoung LEE*,**
*Division of Environmental Health Sciences, College of Public Health, The Ohio State University, 1841 Neil Ave, Columbus, Ohio, 43210, USA
**Department of Food Science and Technology, The Ohio State University, 2015 Fyffe Rd, Columbus, Ohio, 43210, USA
**Department of Food Science and Technology, The Ohio State University, 2015 Fyffe Rd, Columbus, Ohio, 43210, USA
We present an innovative concept of a screening tool for detecting free microcystin in cyanobacteria using a sandwich immunodetection format, based on Michael addition reaction between α,β-unsaturated carbonyl moiety of microcystin and thiol of coating substance. This proof-of-concept immunoassay was developed using bovine serum albumin as a microcystin-binding model, and was tested with toxic Microcystis samples. The preliminary results indicate that the proposed Michael addition-based immunodetection is promising and can be used as a platform for further development to become a useful tool for free microcystin analysis in various samples in the future.
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