Abstract − Analytical Sciences, 34(9), 1055 (2018).
Mass Spectrometric Imaging of GABA in the Drosophila melanogaster Adult Head
Yosuke ENOMOTO,* Phan NT. AN,** Masamitsu YAMAGUCHI,*** Eiichiro FUKUSAKI,* and Shuichi SHIMMA*
*Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
**Institute of Applied Microbiology, Aachen Biology and Biotechnology, RWTH Aachen University, Worringer Weg 1, 52074, Aachen, Germany
***Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo, Kyoto 606-8585, Japan
**Institute of Applied Microbiology, Aachen Biology and Biotechnology, RWTH Aachen University, Worringer Weg 1, 52074, Aachen, Germany
***Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo, Kyoto 606-8585, Japan
Drosophila melanogaster is a model organism in neurodegenerative disease research. In neurodegenerative study, the direct spatial information of neurotransmitters such as γ-aminobutyric acid (GABA) in the brain of Drosophila melanogaster is important to understand the role of GABA. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is an attractive method for direct visualization of neurotransmitters. In this paper, we describe methods to visualize GABA in the brain and head of Drosophila melanogaster using MALDI-IMS.
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