Analytical Sciences

Impact of Saliva Collection and Processing Methods on Aspartate Aminotransferase, Creatin Kinase and Lactate Dehydrogenase Activities

Tomás BARRANCO,*1 Jose J. CERÓN,*1 Pía LÓPEZ-JORNET,*1 Josep PASTOR,*2 Jose M. CARRILLO,*3 Mónica RUBIO,*3 Pedro L. TORNEL,*4 Ramón CUGAT,*5 Fernando TECLES,*1 and Asta TVARIJONAVICIUTE*1

*1 Interdisciplinary Laboratory, Interlab-UMU, Campus of Excellence Mare Nostrum, University of Murcia, Spain
*2 Departament de Medicina i Cirugia Animals, Universidad Autónoma de Barcelona, Barcelona, Spain
*3 Department of Animal Medicine and Surgery, Universidad CEU Cardenal Herrera, Edificio Seminario s/n, 46113, Moncada, Valencia, Spain
*4 Clinical Analysis, University Hospital “Virgen de la Arixaca”, Murcia, Spain
*5 Department of Orthopaedic Surgery and Traumatology, Hospital Quirón, Plaça d’Alfonso Comin, 5-7, 08023, Barcelona, Spain

We aimed to investigate the impact of saliva collection and processing methods on AST, CK and LDH. Saliva was collected from 17 healthy participants by a passive drool. Each saliva sample was distributed into 3 aliquots: not treated, centrifuged, and passed through cotton. Centrifugation improved the precision of assays and produced lower values of AST and CK. The use of cotton resulted in decreased levels of LDH. This data stress the importance of the standardization of sample processing to measure enzymes in saliva.

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