Analytical Sciences

Development of an Immunosensor Based on Surface Plasmon Resonance for Simultaneous Residue Analysis of Three Pesticides —Boscalid, Clothianidin, and Nitenpyram— in Vegetables

Yuki HIRAKAWA,*1,*2 Tomomi YAMASAKI,*1,*2 Ayako HARADA,*3 Seiji IWASA,*3 Hiroshi NARITA,*2 and Shiro MIYAKE*1,*4

*1 Advanced Science, Technology & Management Research Institute of Kyoto, Shimogyo, Kyoto 600-8813, Japan
*2 Kyoto Women’s University, Higashiyama, Kyoto 605-8501, Japan
*3 Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan
*4 Advanced R&D Center, HORIBA, Ltd., Minami, Kyoto 601-8510, Japan

A simultaneous immunosensor based on surface plasmon resonance (SPR) was developed for determination of 3 pesticides —boscalid, clothianidin and nitenpyram— instead of the direct competitive enzyme-linked immunosorbent assays (dcELISAs) widely used as individual determination methods. Carboxy groups that introduced compounds to their pesticides were designed, and conjugates of them and bovine serum albumin were immobilized onto separate channels of the same sensor chip. When a mixture of 3 monoclonal antibodies reacted to each pesticide, and 3 pesticides were injected into the SPR immunosensor, each channel showed specific reactivity at 15 – 93 ng mL⁻¹ for boscalid, 6.7 – 27 ng mL⁻¹ for clothianidin, and 7.3 – 62 ng mL⁻¹ for nitenpyram. Recovery tests using vegetables spiked with a mixture of 3 pesticides showed good results: 75 – 90%, 88 – 104%, and 72 – 105%, respectively, with a high correlation to results of the dcELISAs. The SPR immunosensor would be useful for the determination of pesticide residues in vegetables.

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