Analytical Sciences

An Ascorbic Acid Oxidase-based Sensing Platform for Stereoselective Interaction with Ascorbic Acid and Isoascorbic Acid

Jiao MA,* Juanjuan XU,** Chengcheng YANG,* Jinyi SONG,* and Yingzi FU*

*Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing, China
**Mianyang Product Quality Supervision & Inspection Institute, 621000 Mianyang, China

A simple enzyme-based nanohybrid material was fabricated via immobilizing ascorbic acid oxidase (AO) on the surface of flower-like electrodeposited gold nanoparticles (dpAu) and reduced graphene oxide (rGO) modified glassy carbon electrodes (GCEs). The composite material was used for stereoselective interaction with ascorbic acid (AA) and isoascorbic acid (IAA). Herein, AO was applied as a stereoselective selector, and the dpAu/rGO nanohybrid not only acted as a supporter for high loading of AO, but also served as the nanomaterial for signal amplification. The results showed obvious peak current differences between AA and IAA, indicating that this strategy could be employed to recognize AA and IAA. Under the optimum conditions, the sensor exhibited a good linear response to AA and IAA in a linear range of 1.0 × 10⁻⁴ – 5.0 × 10⁻³ M. This approach with the merits of simplicity and rapid response provided a promising perspective for identification of AA and IAA.

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