Analytical Sciences

Rapid Enzyme-linked Immunosorbent Assays for Diagnosis of Diabetes in a Compact Disc-shaped Microfluidic Device

Shunsuke FURUTANI,*,** Keiko NISHIO,* Nahoko NARUISHI,* Yoko AKAZAWA-OGAWA,* Yoshihisa HAGIHARA,* Yasukazu YOSHIDA,*** and Hidenori NAGAI*,**

*Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan
**Advanced Photonics and Biosensing Open Innovation Laboratory (PhotoBIO-OIL), National Institute of AIST, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
***Health Research Institute, National Institute of AIST, 2217-14 Hayashi, Takamatsu, Kagawa 761-0395, Japan

We have developed a compact disc (CD)-shaped microfluidic device for multiple, rapid enzyme-linked immunosorbent assays (ELISA). The device has a versatile design that can be adapted for the detection of various proteins by selecting the push-in-type reaction parts and appropriate reagents for each target. In this paper, we report the rapid quantification of insulin, adiponectin, and leptin, which can be used for the early diagnosis of diabetes, in human serum in only 16 min with our device.

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