Abstract − Analytical Sciences, 34(3), 259 (2018).
A Label-free and Highly Sensitive Fluorescence Strategy for Mercury Ion Detection Based on Exonuclease III-aided Recycling Amplification
Baomiao DING,* Chenguang LIU,** Qinghua WU,* Yun WANG,** Li LI,** and Hualin YANG**
*Jingchu Food Research and Development Center, College of Life Science, Yangtze University, Jingzhou, Hubei 434025, China
**Edible and Medicinal Fungi Research Center, College of Life Science, Yangtze University, Jingzhou, Hubei 434025, China
**Edible and Medicinal Fungi Research Center, College of Life Science, Yangtze University, Jingzhou, Hubei 434025, China
In this paper, we describe a simple and highly sensitive fluorescence strategy of mercury ions based on exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. With an ultra high sensitivity (1 pM), our strategy has been simple and cost-effective, which does not need any artificial modification fluorescence groups, and can be carried out in a pot. It also shows excellent selectivity. Therefore, our new method provides an effective platform for mercury-ion detection.
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