Abstract − Analytical Sciences, 34(12), 1427 (2018).
Simultaneous Detection of Phosphoproteins and Total Proteins in SDS-PAGE Using Calcon
Sun-Young HWANG and Jung-Kap CHOI
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 61186, South Korea
A novel fluorescent staining protocol to detect phosphoproteins in sodium dodecyl sulfate-polyacrylamide gels using a fluorescence sensor, 1-(2-hydroxy-1-naphthylazo)-2-naphthol-4-sulfonic acid sodium salt (Calcon), was developed. This method yields results within 135 min, with the sensitivities of 15 ng of α-casein and β-casein, and 62.5 ng of κ-casein, respectively. Since non-phosphoproteins have shown negative signals that are distinctly different from positive signals of phosphoproteins, this detection method allows one to monitor phosphoproteins with high specificity. Furthermore, a total protein profile can be achieved before a destaining step using a scanner with rapid and low-cost without further total protein staining.
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