Analytical Sciences

Trace Determination of Cyanocobalamin (Vitamin B₁₂) by Analyzing Cobalt Using a Portable Total-Reflection X-ray Fluorescence Spectrometer

Shinsuke KUNIMURA, Yoshie TOKUOKA, and Umina AONO

Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan

A method is presented for the detection of a trace amount of cyanocobalamin (vitamin B₁₂) using a combination of solid-phase extraction and the measurement of cobalt in vitamin B₁₂ by a portable total-reflection X-ray fluorescence spectrometer. Using this combination, a detection limit of 1 ng/mL was achieved for vitamin B₁₂.

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