Analytical Sciences

Abstract − Analytical Sciences, 33(8), 911 (2017).

A Reactive Oxygen/Nitrogen Species Sensor Fabricated from an Electrode Modified with a Polymerized Iron Porphyrin and a Polymer Electrolyte Membrane
Ryo MATSUOKA,*1 Chihiro KOBAYASHI,*2 Atsushi NAKAGAWA,*2 Shigeo AOYAGI,*1 Tatsuo AIKAWA,*2 Takeshi KONDO,*2,*3 Shigenobu KASAI,*4,*5 and Makoto YUASA*2,*3
*1 HOKUTO DENKO CORPORATION, 3028 Kamiechi, Atsugi, Kanagawa 243-0801, Japan
*2 Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan
*3 Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan
*4 Graduate Department of Environmental Information Engineering, Tohoku Institute of Technology, Sendai 982-8577, Japan
*5 Biomedical Engineering Research Center, Tohoku Institute of Technology, Sendai 982-8577, Japan
We have developed an electrochemical reactive oxygen/nitrogen species sensor that can detect superoxide anion radicals (O2−•) and nitric oxide (NO). The reactive oxygen/nitrogen species sensor was fabricated by surface modification of an electrode with polymerized iron tetrakis(3-thienyl)porphyrin (FeT3ThP), and it can detect either O2−• or NO by switching the applied potential. Furthermore, we fabricated a sensor with improved selectivity by coating a Nafion® film onto the poly(FeT3ThP)-modified electrode. An interference current caused by NO2− was seen for the poly(FeT3ThP)-modified electrode, while the interference current was significantly reduced at the Nafion®/poly(FeT3ThP)-modified electrode, leading to improved selectivity for NO detection. The current response at the Nafion®/poly(FeT3ThP)-modified electrode exhibited good linearity in the O2−• and NO concentration ranges 1.3 – 4.1, and 0.5 – 10 μM, respectively. The Nafion®/poly(FeT3ThP)-modified and poly(FeT3ThP)-modified electrodes are highly versatile, because these electrodes can detect either O2−• or NO by switching the applied potential. Since the Nafion®/poly(FeT3ThP)-modified and poly(FeT3ThP)-modified electrodes contain no bio-derived compounds, the reactive oxygen/nitrogen species sensor should be safe even when it is used in vivo.