Analytical Sciences

Infant Exposure to Bisphenol A Can Be Quantitatively Assessed by a Simply Improved High-Performance Liquid Chromatography–Tandem Mass-Spectrometry Method

Heng WANG,*,**,*** Liangpo LIU,*,** Syed Ali Musstjab Akber Shah EQANI,* and Heqing SHEN*,**

*Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
**University of Chinese Academy of Sciences, Beijing 100049, China
***Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan Municipal Center for Disease Control and Prevention, Zhejiang, Zhoushan 316021, China

To quantitatively measure trace levels of bisphenol A (BPA) in infant urine, a simply improved high-performance liquid chromatography (HPLC)–tandem mass-spectrometry method was developed and validated. In the present work, a ZORBAX SB-C₁₈ column (2.1 × 30 mm, 3.5 μm) was used for trapping and isolating BPA from the HPLC mobile phase. The result showed that the trapping column can provide effective separation from the background BPA to the sample BPA. This method has a limit of detection (LOD) of 0.048 μg/L, spiked recoveries 85.4% (0.50 – 10.0 μg/L), and relative standard deviations of 1.8 – 15.9%. By using its low-LOD advantage, we firstly reported that Chinese infants (n = 48 with age <6 months) have been ubiquitously exposed to BPA (detection frequency of 93%) with an median level of 0.13 μg/L (ranging from below LOD to 5.04 μg/L).

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