Analytical Sciences

Sensitive Method for the Oxidation-determination of Trace Hydroxylamine in Environmental Water Using Hypochlorite Followed by Gas Chromatography

Toshikuni KATO,*1,*2 Shogo SUGAHARA,*1 Makoto MURAKAMI,*1 Yukiko SENGA,*3 Michiko EGAWA,*1 Hiroshi KAMIYA,*2,*4 Kohji OMATA,*1 and Yasushi SEIKE*1,*4

*1 Graduate School of Science and Engineering, Shimane University, Matsue, Shimane 690-8504, Japan
*2 Shimane Prefectural Institute of Public Health and Environmental Science, Matsue, Shimane 690-0823, Japan
*3 Faculty of Science, Toho University, Funabashi, Chiba 274-8510, Japan
*4 Estuary Research Center, Shimane University, Matsue, Shimane 690-8504, Japan

We developed a method for quantifying trace NH₂OH in brackish- and sea-water samples. Previously reported methods applicable to fresh water cannot be applied to such samples. We determined that interference in seawater owing to the bromide ion can be removed by the addition of phenol. In our procedure, phenol and hypochlorite solutions were added to a sample solution to oxidize NH₂OH to N₂O. N₂O in the sample was then quantified by headspace analysis. The method is not affected by the salt content or ammonia, nitrate, or nitrite at concentrations of 300 μgN L⁻¹ or less. It has a limit of detection of 0.2 μgN L⁻¹, and can quantify NH₂OH in natural water samples with a wide range of salinity. It was applied to samples from Lake Nakaumi, a brackish lake located in the eastern part of Shimane Prefecture, Japan.

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