Analytical Sciences

Nanoporous Waveguide Spectroscopy for the Estimation of Enzyme Adsorption on Mesoporous Silica

Hiroyuki ARAFUNE,*1 Kazuhiro HOTTA,*2 Tetsuji ITOH,*3 Norio TERAMAE,*2 and Akira YAMAGUCHI*4

*1 Department of Creative Engineering, National Institute of Technology, Tsuruoka College, 104 Sawada, Inooka, Tsuruoka 997-8511, Japan
*2 Department of Chemistry, Graduate School of Science, Tohoku University, Aoba, Sendai 980-8578, Japan
*3 Research Center for Compact Chemical System, National Institute of Advanced Industrial Science and Technology (AIST), 4-2-1 Nigatake, Miyagino, Sendai 983-8551, Japan
*4 College of Science, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan

Mesoporous silica is considered as promising host material for enzymes due to its uniform pore size of enzyme dimensions and tunable surface chemical properties. In this study, we applied nanoporous waveguide (NPWG) spectroscopy to observe adsorption dynamics of heme proteins with different molecular size within mesoporous silica film modified with different surface functional groups. Since NPWG spectroscopy provides kinetic information and rough quantification of adsorption amount, it is useful to study the adsorption process of enzymes within inorganic nanoporous materials.

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