Analytical Sciences

In situ Observation of Direct Electron Transfer Reaction of Cytochrome c Immobilized on ITO Electrode Modified with 11-{2-[2-(2-Methoxyethoxy)ethoxy]ethoxy}undecylphosphonic Acid Self-assembled Monolayer Film by Electrochemical Slab Optical Waveguide Spectroscopy

Naoki MATSUDA,* Hirotaka OKABE,* Ayako OMURA,** Miki NAKANO,** and Koji MIYAKE**

*Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Japan, 807-1 Shukumachi, Tosu, Saga 841-0052, Japan
**Advanced Manufacturing Research Institute, AIST, Japan, 1-2-1 Namiki, Tsukuba, Ibaraki 305-8564, Japan

To immobilize cytochrome c (cyt.c) on an ITO electrode while keeping its direct electron transfer (DET) functionality, the ITO electrode surface was modified with 11-{2-[2-(2-methoxyethoxy)ethoxy]ethoxy}undecylphosphonic acid (CH₃O (CH₂CH₂O)₃C₁₁H₂₂PO(OH)₂, M-EG₃-UPA) self-assembled monolayer (SAM) film. After a 100-times washing process to exchange a phosphate buffer saline solution surrounding cyt.c and ITO electrode to a fresh one, an in situ observation of visible absorption spectral change with slab optical waveguide (SOWG) spectroscopy showed that 87.7% of the cyt.c adsorbed on the M-EG₃-UPA modified ITO electrode remained on the ITO electrode. The SOWG absorption spectra corresponding to oxidized and reduced cyt.c were observed with setting the ITO electrode potential at 0.3 and –0.3 V vs. Ag/AgCl, respectively, while probing the DET reaction between cyt.c and ITO electrode occurred. The amount of cyt.c was evaluated to be about 19.4% of a monolayer coverage based on the coulomb amount in oxidation and reduction peaks on cyclic voltammetry (CV) data. The CV peak current maintained to be 83.4% compared with the initial value for a M-EG₃-UPA modified ITO electrode after 60 min continuous scan with 0.1 V/s between 0.3 and –0.3 V vs. Ag/AgCl.

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