Analytical Sciences

Luminol Chemiluminescence Profile of O/W Emulsions during Thermal Oxidation

Mitsuhiro WADA,*1 Akiko YAMAGUCHI,*2 Akihiro OGAWA,*3 Hirotsugu KIDO,*4 Shinichi NAKAMURA,*1 Makoto TAKADA,*5 Shigeru KAWAKAMI,*2 Naotaka KURODA,*2 and Kenichiro NAKASHIMA*4

*1 School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-machi, Nobeoka, Miyazaki 882-8508, Japan
*2 Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan
*3 Mitsubishi-Kagaku Foods Co., 1-1-1 Marunouchi, Chiyoda, Tokyo 100-8252, Japan
*4 Aoba Kasei Co., 1-5-6 Oroshi-machi, Wakabayashi, Sendai, Miyagi 984-8642, Japan
*5 Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan

The luminol chemiluminescence (CL) profile of an oil-in-water (O/W) emulsion during thermal oxidation (60°C) was assessed using the luminol-K₃[Fe(CN)₆] assay, in which the oxidation species produced by the autoxidation of an O/W emulsion generated CL emission. Increased CL intensity was observed for O/W emulsions prepared using either linseed or corn oil, which was increased by the addition of Fe²⁺ to the O/W emulsion. The relationship between the CL profile and results obtained by conventional approaches, such as the peroxide value (PV) and thiobarbituric acid (TBA) methods, were compared. Owing to good correlation between the CL intensity and results obtained by the methods, the CL method might be applicable for estimating the oil oxidizing of an emulsion in thermal oxidation.

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