Abstract − Analytical Sciences, 33(1), 9 (2017).
A Label-free and Turn-on Fluorescence Strategy for DNA Detection with a Wide Detection Range Based on Exonuclease III-aided Target Recycling Amplification
Hualin YANG,* Xiaoda SONG,** Baomiao DING,* Zhenshun LI,* and Xingping ZHANG*
*Edible and Medicinal Fungi Research Center, College of Life Science, Yangtze University, Jingzhou, Hubei 434025, China
**School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, China
**School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, China
In the present work, a simple and sensitive turn-on fluorescence method for DNA detection was developed. It was explored based on the N-methyl mesoporphyrin IX (NMM)/G-quadruplex DNA system as a reporter and exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. Our method showed an ultra-wide detection range from 10 fM to 100 nM with a low linear detection limit of 0.76 nM. It also had excellent selectivity in a selectivity experiment.
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