Analytical Sciences

Pneumocandin B₀-imprinted Polymer Using Surface-imprinting Technique for Efficient Purification of Crude Product

Lu ZHANG,*1 Hua ZHANG,*1 Le LI,*1 Peng ZUO,*2 Feilang ZHAO,*3 Manhua LIU,*1 Bang-Ce YE,*2,*4 and Yingchun LI*1,*3,*4

*1 Key Laboratory of Xinjiang Phytomedicine Resources for Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, China
*2 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
*3 Jiangsu Devote Instrumental Science & Technology Co., Ltd., Huai’an, Jiangsu 223001, China
*4 Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China

In this work, we prepared surface molecularly imprinted polymer (MIP) for selective recognition of pneumocandin B₀ (PNB₀). Methacrylic acid (MAA) was first grafted onto silica gel particles (SiO₂) in the manner of “grafting from” by using 3-methacryloxypropyl trimethoxysilane as intermedium, and then PNB₀ molecules were imprinted on the surface of the obtained particles in the presence of ethylene glycol diglycidyl ether as the cross-linker. The prepared MIP-PMAA/SiO₂ was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and thermo-gravimetric analysis, which confirmed the successful grafting of MAA onto SiO₂ and the grafting degree was calculated to be 12.50 wt%. The binding properties of the products were investigated and it is found that the binding process of PNB₀ followed the pseudo-second-order kinetic model. The as-prepared material also displays relatively quick adsorption kinetics and decent recognition affinity toward the template over its structurally related compound.

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