Analytical Sciences

Investigation of 5-(3-Trifluoromethylbenzylidene)thiazolidine-2,4-dione as a Matrix for Analyses of Biogenic Monoamine Transmitters Using MALDI-MS

Hiroko KASAI,*1 Masamichi NAKAKOSHI,*2 Tomomi SUGITA,*3 Mayu MATSUOKA,*3 Yuzo YAMAZAKI,*4 Yumi UNNO,*4 Hiroki NAKAJIMA,*4 Hideshi FUJIWAKE,*4 and Masayoshi TSUBUKI*3

*1 Central Research Laboratory, Hoshi University, 2-4-41 Ebara, Shinagawa, Tokyo 142-8501, Japan
*2 Department of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
*3 Institute of Medicinal Chemistry, Hoshi University, 2-4-41 Ebara, Shinagawa, Tokyo 142-8501, Japan
*4 Global Application Development Center, Shimadzu Corporation, 1 Nishinokyo Kuwabara-cho, Nakagyo, Kyoto 604-8511, Japan

In order to discover new matrices suitable for the analyses of low molecular-weight compounds using positive-ion mode matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (MS), 5-(3-trifluoromethylbenzylidene)thiazolidine-2,4-dione (3-CF₃-BTD) was synthesized, and its effectiveness was compared with that when commercially available α-cyano-4-hydroxycinnamic acid was used. 3-CF₃-BTD was sufficiently sensitive to analyze neurotransmitters, i.e., dopamine, serotonin, histamine, and epinephrine, in amounts of several picomoles. Similar to vacuum MALDI experiments, atmospheric-pressure MALDI-MS measurements using 3-CF₃-BTD as a matrix also detected dopamine.

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